Guanidine Interference in Determination of Creatine Kinase
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چکیده
منابع مشابه
NOVEL REAGENTS FOR CREATINE KINASE
The purpose of this investigation was to develop a simple colorimetric method for creatine kinase (CK). The new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with different glyoxal compounds. Hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were...
متن کاملRare interference in determinations of total creatine kinase.
16518-23. 4. Kohara K, Tabuchi Y, Senanayake P. Brosnihan KB, Ferrario CM. Reassessment of plasma angiotensins measurement: effects of pro. tease inhibitors and sample handling procedures. Peptides 1991:12:1135-41. 5. Welches WR, Santos RAS, Chappell MC, Brosnihan KB, Greene U, Ferrario CM. Evidence that prolyl endopeptidase participates in the processing of brain angiotensin. I Hypertens 1991:...
متن کاملDetermination of creatine, creatinine, arginine, guanidinoacetic acid, guanidine, and methylguanidine in biological fluids.
The non-specific analytical methods for the guanidinium compounds in biological fluids have been unsatisfactory for many years. The methods presented in this paper have a greatly improved specificity and are not too complex for use in the clinical laboratory. A modified Sakaguchi color reaction for substituted guanidines is used in each procedure. Guanidinoacetic acid is measured by developing ...
متن کاملCreatine kinase in serum: 1. Determination of optimum reaction conditions.
To establish optimum conditions for creatine kinase (EC 2.7.3.2) activity measurement with the creatine phosphate in equilibrium creatine reaction, we re-examined all kinetics factors relevant to an optimal and standardized enzyme assay at 30 and 25 degrees C. We determined the pH optimum in vaious buffers, considering the effect of the type and concentration of the buffer, as well as the influ...
متن کاملCreatine kinase in serum: 2. Interference of adenylate kinase with the assay.
Interference of adenylate kinase with Oliver's method [Biochem. J. 61, 116 (1955)] for creatine kinase is usually suppressed by including an adenylate kinase inhibitor, AMP. We studied the kinetics and compared the inhibition capacities of AMP and diadenosine pentaphosphate. Both are competitive inhibitors, AMP being markedly weaker, with a Ki of about 300 mumol/liter for adenylate kinase from ...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1975
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/21.1.168